Separation of the pigments in the mint leaves

Paper chromatography is the technique used to separate the compounds of a mixture taking the advantage of their different polarity. The following pictures show how the solvent (mobile phase) carries the pigments through the stationary phase (paper).




The retention factor can be calculated dividing the distance travelled by the spots (a) by the distance travelled by the solvent (b).



The following picture shows the chromatogram with two spots (green and yellow) corresponding to chlorophyll and xanthophyll compounds with their retention factors depending on their affinity to the mobile phase. 


Read the text The colour of leaves and answer the qüestions:

Which are the main pigments present in the leaves?

Which pigment is the responsible for the green colour of leaves?

Which pigment is the responsible for the yellow colour of leaves?

Which pigment is the responsible for the brown colour of leaves?

Which are the two parts of chlorophyll molecule?


Which part of the structure of the chlorophyll molecule is the responsible of the absorption of light?

Make the quiz you can find at the end of the Paper chromatography webpage.

Comentaris

  1. Juan Palazon6/10/23 2:20 a. m.

    1.Chlorophylls, carotenoids and anthocyanins
    2.Chlorophyll
    3.Carotenoids
    4.Anthocyanins
    5.Porphyrin nucleus and hydrophobic tail
    6.Conjugated double bonds

    ResponElimina
  2. Guillem Reixach6/10/23 2:20 a. m.

    1. Chlorophylls, carotenoids and anthocyanins
    2. Chlorophyll
    3. Carotenoids
    4. Anthocyanins
    5. Porphyrin nucleus and hydrophobic tail
    6. Conjgated double bonds

    ResponElimina
  3. Anònim6/10/23 2:20 a. m.

    1. Chlorophyll and xanthophyll

    2.Chlorphyll

    3.pheophytin

    4.phorphyrin and magnesium

    5.chlorophyll

    ResponElimina
  4. nereas6/10/23 2:28 a. m.

    1)the main pigments are chlorophylls, carotenoids and anthocyanins.
    2) the Chlorophyll
    3) xanthophylls, a subclass of carotenoids
    4)Antocyanins and carotenoids
    5) orange is carotenoids
    6) a porphyrin nucleus, containing nitrogen molecules, similar to that of the haemoglobin in our blood and the hydrophobic tail with 20 carbon atoms, phytol
    7) the presence of numerous conjugated double bonds in its structure

    ResponElimina
  5. Anònim6/10/23 2:29 a. m.

    Emma

    Which are the main pigments present in the leaves?
    -Plants produce an incredible diversity of pigment.
    There are several types of pigments in leaves, mostly chlorophylls, carotenoids and anthocyanins.

    Which pigment is the responsible for the green colour of leaves?
    -responsible for the green color of the leaves is the chlorophylls.

    Which pigment is the responsible for the yellow colour of leaves?
    -the pigment responsible for the yellow leaf is the carotenoids and anthocyanins.

    ResponElimina
  6. Anònim6/10/23 2:35 a. m.

    Which are the main pigments present in the leaves?
    They are chlorophylls, carotenoids and anthocyanins.

    Which pigment is the responsible for the green colour of leaves?
    Chlorophyll is responsible.

    Which pigment is the responsible for the yellow colour of leaves?
    Carotenoids is responsible

    Which pigment is the responsible for the brown colour of leaves?
    Anthocyanins is responsible

    Which are the two parts of chlorophyll molecule?
    There are porphyrin nucleus and hydrophobic

    Which part of the structure of the chlorophyll molecule is the responsible of the absorption of light?
    It was for a numerous conjugated of double bonds

    ResponElimina
  7. Carla i Aina6/10/23 2:35 a. m.

    Which are the main pigments present in the leaves?
    - The main pigments present in leaves are: chlorophylls, carotenoids and anthocyanins.

    Which pigment is the responsible for the green colour of leaves?
    - The responsible pigment for the green colour leaves is the chlorophyll.

    Which pigment is the responsible for the yellow colour of leaves?
    - Carotenoids and flavonoids are the responsible pigements for the yellow colour of leaves.

    Wich pigment is the responsible for the orange colour of leaves?
    - The responsible pigments for the orange colour of leaves are carotenoids

    Which pigment is the responsible for the brown colour of leaves?
    -Anthocyanins and carotenoids are the responsible pigments for the brown colour of leaves.

    Which are the two parts of chlorophyll molecule?
    - The two parts of clhorophyill molecule are porphyrin nucleus and hydrophobic tail.

    Which part of the structure of the chlorophyll molecule is the responsible of the absorption of light?
    - The part of the structure of the chlorophyll molecule are the conjugated double bonds.

    ResponElimina

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